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SOLVED: Using the equation for calculating the melting temperature (Tm) for primers, calculate the Tm's for each primer in degrees Celsius. Apart from the melting temperature of the primers, describe two other
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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
Primer sequences and annealing temperature (Tm) for amplification and... | Download Table
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